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X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily.

Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM
EMBO J 19 5269-80 (2000)
Related entries: 1fo9, 1foa

Cited: 140 times
EuropePMC logo PMID: 11032794

Abstract

N:-acetylglucosaminyltransferase I (GnT I) serves as the gateway from oligomannose to hybrid and complex N:-glycans and plays a critical role in mammalian development and possibly all metazoans. We have determined the X-ray crystal structure of the catalytic fragment of GnT I in the absence and presence of bound UDP-GlcNAc/Mn(2+) at 1.5 and 1.8 A resolution, respectively. The structures identify residues critical for substrate binding and catalysis and provide evidence for similarity, at the mechanistic level, to the deglycosylation step of retaining beta-glycosidases. The structuring of a 13 residue loop, resulting from UDP-GlcNAc/Mn(2+) binding, provides an explanation for the ordered sequential 'Bi Bi' kinetics shown by GnT I. Analysis reveals a domain shared with Bacillus subtilis glycosyltransferase SpsA, bovine beta-1,4-galactosyl transferase 1 and Escherichia coli N:-acetylglucosamine-1-phosphate uridyltransferase. The low sequence identity, conserved fold and related functional features shown by this domain define a superfamily whose members probably share a common ancestor. Sequence analysis and protein threading show that the domain is represented in proteins from several glycosyltransferase families.

Reviews - 1fo8 mentioned but not cited (2)

  1. Replication and partitioning of papillomavirus genomes. McBride AA. Adv. Virus Res. 72 155-205 (2008)
  2. 3D Structure and Function of Glycosyltransferases Involved in N-glycan Maturation. Nagae M, Yamaguchi Y, Taniguchi N, Kizuka Y. Int J Mol Sci 21 (2020)

Articles - 1fo8 mentioned but not cited (5)

  1. X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: catalytic mechanism and a new protein superfamily. Unligil UM, Zhou S, Yuwaraj S, Sarkar M, Schachter H, Rini JM. EMBO J. 19 5269-5280 (2000)
  2. Bovine alpha1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases. Gastinel LN, Bignon C, Misra AK, Hindsgaul O, Shaper JH, Joziasse DH. EMBO J. 20 638-649 (2001)
  3. The tail sheath structure of bacteriophage T4: a molecular machine for infecting bacteria. Aksyuk AA, Leiman PG, Kurochkina LP, Shneider MM, Kostyuchenko VA, Mesyanzhinov VV, Rossmann MG. EMBO J. 28 821-829 (2009)
  4. Identification of putative rhamnogalacturonan-II specific glycosyltransferases in Arabidopsis using a combination of bioinformatics approaches. Voxeur A, André A, Breton C, Lerouge P. PLoS ONE 7 e51129 (2012)
  5. Structure-function features of a Mycoplasma glycolipid synthase derived from structural data integration, molecular simulations, and mutational analysis. Romero-García J, Francisco C, Biarnés X, Planas A. PLoS One 8 e81990 (2013)


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