› 1fzj

X-ray diffraction
1.9Å resolution

MHC CLASS I NATURAL MUTANT H-2KBM1 HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN

Released:
DOI: 10.2210/pdb1fzj/pdb
PDB entry 1fzj coloured by chain, front view.
PDB entry 1fzj coloured by chain, side view.
PDB entry 1fzj coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structures of K(bm1) and K(bm8) reveal that subtle changes in the peptide environment impact thermostability and alloreactivity.
Rudolph MG, Speir JA, Brunmark A, Mattsson N, Jackson MR, Peterson PA, Teyton L, Wilson IA
Immunity 14 231-42 (2001)
PMID: 11290333

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
PDBe Complex ID:
PDB-CPX-135046 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.66 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Nucleoprotein Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 956 Da
Source organism: Vesicular stomatitis Indiana virus strain Glasgow
Expression system: Not provided
UniProt:
  • Canonical: P11212 (Residues: 52-59; Coverage: 2%)
Gene name: N

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUL
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand PO4 2 x PO4
Ligand MPD 3 x MPD
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21212
Unit cell:
a: 134.91Å b: 88.35Å c: 45.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.222
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

8 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
7 more

4 mentions without citation

Structural comparison of allogeneic and syngeneic T cell receptor-peptide-major histocompatibility complex complexes: a buried alloreactive mutation subtly alters peptide presentation substantially increasing V(beta) Interactions.
Luz et al. (2002)
3 more