› 1g5c

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE 'CAB' TYPE BETA CLASS CARBONIC ANHYDRASE FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

Released:
DOI: 10.2210/pdb1g5c/pdb
PDB entry 1g5c coloured by chain, front view.
PDB entry 1g5c coloured by chain, side view.
PDB entry 1g5c coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Crystal structure of the "cab"-type beta class carbonic anhydrase from the archaeon Methanobacterium thermoautotrophicum.
Strop P, Smith KS, Iverson TM, Ferry JG, Rees DC
J Biol Chem 276 10299-305 (2001)
PMID: 11096105

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 170 amino acids
Theoretical weight: 18.87 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q50565 (Residues: 6-175; Coverage: 97%)
Gene name: MTH_1582
Sequence domains: Carbonic anhydrase
Structure domains: Carbonic anhydrase

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand CA 6 x CA
Ligand EPE 3 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 54.67Å b: 113.21Å c: 156.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.248
Expression system: Escherichia coli

Quick links

Citations

14 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
13 more

8 mentions without citation

Thermostable Carbonic Anhydrases in Biotechnological Applications.
Di Fiore et al. (2015)
7 more