› 1g5h

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF THE ACCESSORY SUBUNIT OF MURINE MITOCHONDRIAL POLYMERASE GAMMA

Released:
DOI: 10.2210/pdb1g5h/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1g5h coloured by chain, front view.
PDB entry 1g5h coloured by chain, side view.
PDB entry 1g5h coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure and deletion analysis show that the accessory subunit of mammalian DNA polymerase gamma, Pol gamma B, functions as a homodimer.
Carrodeguas JA, Theis K, Bogenhagen DF, Kisker C
Mol Cell 7 43-54 (2001)
PMID: 11172710

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase subunit gamma-2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 454 amino acids
Theoretical weight: 51.34 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9QZM2 (Residues: 17-459; Coverage: 97%)
Gene names: Mtpolb, Polg2
Sequence domains: Anticodon binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 4 x NA
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25, NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 96.623Å b: 133.422Å c: 135.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.224
Expression system: Escherichia coli

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Citations

28 review citations

Mitochondrial DNA mutations in human disease.
Taylor et al. (2005)
27 more

5 mentions without citation

Mutant POLG2 disrupts DNA polymerase gamma subunits and causes progressive external ophthalmoplegia.
Longley et al. (2006)
4 more