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The structure and oligomerization of the yeast arginine methyltransferase, Hmt1.

Weiss VH, McBride AE, Soriano MA, Filman DJ, Silver PA, Hogle JM
Nat Struct Biol 7 1165-71 (2000)
Cited: 74 times
EuropePMC logo PMID: 11101900

Abstract

Protein methylation at arginines is ubiquitous in eukaryotes and affects signal transduction, gene expression and protein sorting. Hmt1/Rmt1, the major arginine methyltransferase in yeast, catalyzes methylation of arginine residues in several mRNA-binding proteins and facilitates their export from the nucleus. We now report the crystal structure of Hmt1 at 2.9 A resolution. Hmt1 forms a hexamer with approximate 32 symmetry. The surface of the oligomer is dominated by large acidic cavities at the dimer interfaces. Mutation of dimer contact sites eliminates activity of Hmt1 both in vivo and in vitro. Mutating residues in the acidic cavity significantly reduces binding and methylation of the substrate Npl3.

Reviews - 1g6q mentioned but not cited (1)

  1. AdoMet-dependent methylation, DNA methyltransferases and base flipping. Cheng X, Roberts RJ. Nucleic Acids Res 29 3784-3795 (2001)

Articles - 1g6q mentioned but not cited (2)

  1. RNA:(guanine-N2) methyltransferases RsmC/RsmD and their homologs revisited--bioinformatic analysis and prediction of the active site based on the uncharacterized Mj0882 protein structure. Bujnicki JM, Rychlewski L. BMC Bioinformatics 3 10 (2002)
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Reviews citing this publication (16)

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Articles citing this publication (55)

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