› 1g9h

X-ray diffraction
1.8Å resolution

TERNARY COMPLEX BETWEEN PSYCHROPHILIC ALPHA-AMYLASE, COMII (PSEUDO TRI-SACCHARIDE FROM BAYER) AND TRIS (2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL)

Released:
DOI: 10.2210/pdb1g9h/pdb
PDB entry 1g9h coloured by chain, front view.
PDB entry 1g9h coloured by chain, side view.
PDB entry 1g9h coloured by chain, top view.
Source organism: Pseudoalteromonas haloplanktis
Primary publication:
Crystallographic evidence of a transglycosylation reaction: ternary complexes of a psychrophilic alpha-amylase.
Aghajari N, Roth M, Haser R
Biochemistry 41 4273-80 (2002)
PMID: 11914073

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 48.95 KDa
Source organism: Pseudoalteromonas haloplanktis
UniProt:
  • Canonical: P29957 (Residues: 25-472; Coverage: 70%)
Gene name: amy
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, DAF
Ligand BGC 1 x BGC
Ligand DAF 1 x DAF
3 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: C2221
Unit cell:
a: 71.33Å b: 138.68Å c: 114.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.201

Quick links

Citations

5 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
4 more

1 mention without citation

M-ORBIS: mapping of molecular binding sites and surfaces.
Albou et al. (2011)