› 1gei

X-ray diffraction
1.6Å resolution

STRUCTURAL CHARACTERIZATION OF N-BUTYL-ISOCYANIDE COMPLEXES OF CYTOCHROMES P450NOR AND P450CAM

Released:
DOI: 10.2210/pdb1gei/pdb
PDB entry 1gei coloured by chain, front view.
PDB entry 1gei coloured by chain, side view.
PDB entry 1gei coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
Structural characterization of n-butyl-isocyanide complexes of cytochromes P450nor and P450cam.
Lee DS, Park SY, Yamane K, Obayashi E, Hori H, Shiro Y
Biochemistry 40 2669-77 (2001)
PMID: 11258878

Function and Biology Details

Reaction catalysed: 
Nitrous oxide + NAD(P)(+) + H(2)O = 2 nitric oxide + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-540436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP nitrous oxide-forming nitric oxide reductase Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.42 KDa
Source organism: Fusarium oxysporum
Expression system: Escherichia coli
UniProt:
  • Canonical: P23295 (Residues: 1-403; Coverage: 100%)
Gene names: CYP55, CYP55A1
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand NBN 1 x NBN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P212121
Unit cell:
a: 54.75Å b: 81.64Å c: 86.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.258
Expression system: Escherichia coli

Quick links

Citations

12 citation in other articles

Crystal structures of [Fe]-hydrogenase in complex with inhibitory isocyanides: implications for the H2-activation site.
Tamura et al. (2013)
11 more

2 mentions without citation

How does the reductase help to regulate the catalytic cycle of cytochrome P450 3A4 using the conserved water channel?
Fishelovitch et al. (2010)
1 more