› 1ggr

Solution NMR

COMPLEX OF ENZYME IIAGLC AND THE HISTIDINE-CONTAINING PHOSPHOCARRIER PROTEIN HPR FROM ESCHERICHIA COLI NMR, RESTRAINED REGULARIZED MEAN STRUCTURE

Released:
DOI: 10.2210/pdb1ggr/pdb
PDB entry 1ggr coloured by chain, ensemble of 3 models, front view.
PDB entry 1ggr coloured by chain, ensemble of 3 models, side view.
PDB entry 1ggr coloured by chain, ensemble of 3 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure of the phosphoryl transfer complex between the signal transducing proteins HPr and IIA(glucose) of the Escherichia coli phosphoenolpyruvate:sugar phosphotransferase system.
Wang G, Louis JM, Sondej M, Seok YJ, Peterkofsky A, Clore GM
EMBO J 19 5635-49 (2000)
PMID: 11060015

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141945 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system glucose-specific EIIA component Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 18.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69783 (Residues: 2-169; Coverage: 99%)
Gene names: JW2410, b2417, crr, gsr, iex, tgs, treD
Sequence domains: phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 1
Structure domains: Glucose Permease (Domain IIA)
Phosphocarrier protein HPr Chain: B
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.13 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA04 (Residues: 1-85; Coverage: 100%)
Gene names: JW2408, b2415, hpr, ptsH
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like

Ligands and Environments

1 bound ligand:
Ligand PO3 1 x PO3
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: RIGID BODY MINIMIZATION AND CONSTRAINED/RESTRAINED SIMULATED ANNEALING
Expression system: Escherichia coli

Quick links

Citations

10 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
9 more

8 mentions without citation

The ClusPro web server for protein-protein docking.
Kozakov et al. (2017)
7 more