› 1ggu

X-ray diffraction
2.1Å resolution

HUMAN FACTOR XIII WITH CALCIUM BOUND IN THE ION SITE

Released:
DOI: 10.2210/pdb1ggu/pdb
PDB entry 1ggu coloured by chain, front view.
PDB entry 1ggu coloured by chain, side view.
PDB entry 1ggu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of the calcium binding site and a novel ytterbium site in blood coagulation factor XIII by x-ray crystallography.
Fox BA, Yee VC, Pedersen LC, Le Trong I, Bishop PD, Stenkamp RE, Teller DC
J Biol Chem 274 4917-23 (1999)
PMID: 9988734

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coagulation factor XIII A chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 731 amino acids
Theoretical weight: 83.23 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00488 (Residues: 2-732; Coverage: 100%)
Gene names: F13A, F13A1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 100.172Å b: 70.764Å c: 133.822Å
α: 90° β: 106.11° γ: 90°
R-values:
R R work R free
0.227 0.227 0.313
Expression system: Saccharomyces cerevisiae

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Citations

14 review citations

Transglutaminases: crosslinking enzymes with pleiotropic functions.
Lorand et al. (2003)
13 more

10 mentions without citation

Regulation of the activities of the mammalian transglutaminase family of enzymes.
Klöck et al. (2012)
9 more