› 1gsv

X-ray diffraction
1.75Å resolution

Crystal structure of the P65 crystal form of photoactive yellow protein G47S mutant

Released:
DOI: 10.2210/pdb1gsv/pdb
PDB entry 1gsv coloured by chain, front view.
PDB entry 1gsv coloured by chain, side view.
PDB entry 1gsv coloured by chain, top view.
Source organism: Halorhodospira halophila
Primary publication:
Engineering photocycle dynamics. Crystal structures and kinetics of three photoactive yellow protein hinge-bending mutants.
van Aalten DM, Haker A, Hendriks J, Hellingwerf KJ, Joshua-Tor L, Crielaard W
J Biol Chem 277 6463-8 (2002)
PMID: 11714713

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.92 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P65
Unit cell:
a: 40.306Å b: 40.306Å c: 116.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.246
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Identification of six new photoactive yellow proteins--diversity and structure-function relationships in a bacterial blue light photoreceptor.
Kumauchi et al. (2008)
5 more

2 mentions without citation

X-ray vs. NMR structures as templates for computational protein design.
Schneider et al. (2009)
1 more