EC 1.3.1.2: Dihydropyrimidine dehydrogenase (NADP(+))
Reaction catalysed:
5,6-dihydrouracil + NADP(+) = uracil + NADPH
Systematic name:
5,6-dihydrouracil:NADP(+) 5-oxidoreductase
Alternative Name(s):
- 4,5-dihydrothymine: oxidoreductase
- DHPDH
- DHU dehydrogenase
- DPD
- Dehydrogenase, dihydrouracil (nicotinamide adenine dinucleotide phosphate)
- Dihydrothymine dehydrogenase
- Dihydrouracil dehydrogenase (NADP+)
- Hydropyrimidine dehydrogenase
GO terms
Biochemical function:
- 4 iron, 4 sulfur cluster binding
- iron-sulfur cluster binding
- NADP binding
- flavin adenine dinucleotide binding
- metal ion binding
- protein homodimerization activity
- dihydropyrimidine dehydrogenase (NADP+) activity
- oxidoreductase activity, acting on the CH-CH group of donors
- oxidoreductase activity
- FMN binding
- uracil binding
- nucleotide binding
Biological process:
Cellular component:
Sequence families
Protein families (Pfam)
![The deposited structure of PDB entry 1gt8 contains 4 copies of Pfam domain PF14697 (4Fe-4S dicluster domain) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_Pfam_PF14697_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of Pfam domain <span class='highlight'>PF14697 (4Fe-4S dicluster domain)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
![The deposited structure of PDB entry 1gt8 contains 4 copies of Pfam domain PF01180 (Dihydroorotate dehydrogenase) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_Pfam_PF01180_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of Pfam domain <span class='highlight'>PF01180 (Dihydroorotate dehydrogenase)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
![The deposited structure of PDB entry 1gt8 contains 4 copies of Pfam domain PF07992 (Pyridine nucleotide-disulphide oxidoreductase) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_Pfam_PF07992_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of Pfam domain <span class='highlight'>PF07992 (Pyridine nucleotide-disulphide oxidoreductase)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
![The deposited structure of PDB entry 1gt8 contains 4 copies of Pfam domain PF14691 (Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_Pfam_PF14691_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of Pfam domain <span class='highlight'>PF14691 (Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
InterPro annotations
Structure domains
CATH domains
3.50.50.60
Class:
Alpha Beta
Architecture:
3-Layer(bba) Sandwich
Topology:
FAD/NAD(P)-binding domain
Homology:
FAD/NAD(P)-binding domain
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 8 copies of CATH domain 3.50.50.60 (FAD/NAD(P)-binding domain) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 2 copies in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_CATH_3.50.50.60_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 8 copies of CATH domain <span class='highlight'>3.50.50.60 (FAD/NAD(P)-binding domain)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 2 copies in chain <span class='highlight'>A</span>.")
3.30.70.20
Class:
Alpha Beta
Architecture:
2-Layer Sandwich
Topology:
Alpha-Beta Plaits
Homology:
Alpha-Beta Plaits
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of CATH domain 3.30.70.20 (Alpha-Beta Plaits) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_CATH_3.30.70.20_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of CATH domain <span class='highlight'>3.30.70.20 (Alpha-Beta Plaits)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
3.20.20.70
Class:
Alpha Beta
Architecture:
Alpha-Beta Barrel
Topology:
TIM Barrel
Homology:
Aldolase class I
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of CATH domain 3.20.20.70 (TIM Barrel) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_CATH_3.20.20.70_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of CATH domain <span class='highlight'>3.20.20.70 (TIM Barrel)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
![The deposited structure of PDB entry 1gt8 contains 4 copies of CATH domain 1.10.1060.10 (Fumarate Reductase Iron-sulfur Protein; Chain B, domain 2) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_CATH_1.10.1060.10_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of CATH domain <span class='highlight'>1.10.1060.10 (Fumarate Reductase Iron-sulfur Protein; Chain B, domain 2)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
SCOP annotations
51972
Class:
Alpha and beta proteins (a/b)
Fold:
Nucleotide-binding domain
Superfamily:
Nucleotide-binding domain
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of SCOP domain 51972 (N-terminal domain of adrenodoxin reductase-like) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_SCOP_51972_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of SCOP domain <span class='highlight'>51972 (N-terminal domain of adrenodoxin reductase-like)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
54884
Class:
Alpha and beta proteins (a+b)
Fold:
Ferredoxin-like
Superfamily:
4Fe-4S ferredoxins
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of SCOP domain 54884 (Ferredoxin domains from multidomain proteins) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_SCOP_54884_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of SCOP domain <span class='highlight'>54884 (Ferredoxin domains from multidomain proteins)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
51396
Class:
Alpha and beta proteins (a/b)
Fold:
TIM beta/alpha-barrel
Superfamily:
FMN-linked oxidoreductases
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of SCOP domain 51396 (FMN-linked oxidoreductases) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_SCOP_51396_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of SCOP domain <span class='highlight'>51396 (FMN-linked oxidoreductases)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
51906
Class:
Alpha and beta proteins (a/b)
Fold:
FAD/NAD(P)-binding domain
Superfamily:
FAD/NAD(P)-binding domain
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of SCOP domain 51906 (C-terminal domain of adrenodoxin reductase-like) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_SCOP_51906_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of SCOP domain <span class='highlight'>51906 (C-terminal domain of adrenodoxin reductase-like)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")
46553
Class:
All alpha proteins
Fold:
Globin-like
Superfamily:
alpha-helical ferredoxin
Occurring in:
![The deposited structure of PDB entry 1gt8 contains 4 copies of SCOP domain 46553 (Dihydropyrimidine dehydrogenase, N-terminal domain) in Dihydropyrimidine dehydrogenase [NADP(+)]. Showing 1 copy in chain A.](https://www.ebi.ac.uk/pdbe/static/entry/1gt8_1_A_SCOP_46553_image-200x200.png "The deposited structure of PDB entry <span class='highlight'>1gt8</span> contains 4 copies of SCOP domain <span class='highlight'>46553 (Dihydropyrimidine dehydrogenase, N-terminal domain)</span> in <span class='highlight'>Dihydropyrimidine dehydrogenase [NADP(+)]</span>. Showing 1 copy in chain <span class='highlight'>A</span>.")