› 1gu5

X-ray diffraction
2.1Å resolution

Crystal structure of C/EBPBETA BZIP homodimer bound to a DNA fragment from the MIM-1 promoter

Released:
DOI: 10.2210/pdb1gu5/pdb
PDB entry 1gu5 coloured by chain, front view.
PDB entry 1gu5 coloured by chain, side view.
PDB entry 1gu5 coloured by chain, top view.
Source organisms:
Entry authors: Tahirov TH, Ogata K

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-506096 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
CCAAT/enhancer-binding protein beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 9.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 259-336; Coverage: 23%)
Gene names: CEBPB, PP9092, TCF5
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
5'-D(*TP*TP*GP*TP*GP*TP*TP*GP*GP*CP* CP*AP*AP*TP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*AP*TP*GP*AP*TP*TP*GP*GP*CP*CP* AP*AP*CP*AP*CP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: C2221
Unit cell:
a: 101.596Å b: 113.037Å c: 74.498Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.277
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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