› 1gun

X-ray diffraction
1.83Å resolution

MopII from Clostridium pasteurianum complexed with molybdate (partial)

Released:
DOI: 10.2210/pdb1gun/pdb
PDB entry 1gun coloured by chain, front view.
PDB entry 1gun coloured by chain, side view.
PDB entry 1gun coloured by chain, top view.
Source organism: Clostridium pasteurianum
Primary publication:
Passive acquisition of ligand by the MopII molbindin from Clostridium pasteurianum: structures of apo and oxyanion-bound forms.
Schüttelkopf AW, Harrison JA, Boxer DH, Hunter WN
J Biol Chem 277 15013-20 (2002)
PMID: 11836258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdenum-pterin-binding protein 2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 68 amino acids
Theoretical weight: 7.02 KDa
Source organism: Clostridium pasteurianum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08854 (Residues: 1-68; Coverage: 100%)
Gene name: mopII
Sequence domains: TOBE domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand MOO 8 x MOO
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 56.81Å b: 78.38Å c: 95.24Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.216 0.215 0.248
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
2 more

1 mention without citation

Relating destabilizing regions to known functional sites in proteins.
Dessailly et al. (2007)