› 1gvy

X-ray diffraction
1.7Å resolution

Substrate distorsion by beta-mannanase from Pseudomonas cellulosa

Released:
DOI: 10.2210/pdb1gvy/pdb
PDB entry 1gvy coloured by chain, front view.
PDB entry 1gvy coloured by chain, side view.
PDB entry 1gvy coloured by chain, top view.
Source organism: Cellvibrio japonicus
Primary publication:
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state.
Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabó L, Stoll D, Withers SG, Davies GJ
Angew Chem Int Ed Engl 41 2824-7 (2002)
PMID: 12203498

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mannan endo-1,4-beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 383 amino acids
Theoretical weight: 43.33 KDa
Source organism: Cellvibrio japonicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P49424 (Residues: 39-421; Coverage: 97%)
Gene names: CJA_2770, man26A, manA
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: MBF, BMA
Carbohydrate polymer
4 bound ligands:
Ligand NIN 1 x NIN
Ligand ZN 2 x ZN
Ligand NA 1 x NA
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P41
Unit cell:
a: 93.192Å b: 93.192Å c: 54.343Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.145 0.144 0.17
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Microbial hemicellulases.
Shallom et al. (2003)
6 more

3 mentions without citation

Dissecting conformational contributions to glycosidase catalysis and inhibition.
Speciale et al. (2014)
2 more