› 1gwn

X-ray diffraction
2.1Å resolution

The crystal structure of the core domain of RhoE/Rnd3 - a constitutively activated small G protein

Released:
DOI: 10.2210/pdb1gwn/pdb
PDB entry 1gwn coloured by chain, front view.
PDB entry 1gwn coloured by chain, side view.
PDB entry 1gwn coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the core domain of RhoE/Rnd3: a constitutively activated small G protein.
Garavini H, Riento K, Phelan JP, McAlister MS, Ridley AJ, Keep NH
Biochemistry 41 6303-10 (2002)
PMID: 12009891

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho-related GTP-binding protein RhoE Chains: A, C
Molecule details ›
Chains: A, C
Length: 205 amino acids
Theoretical weight: 22.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61588 (Residues: 16-200; Coverage: 76%)
Gene names: Arhe, Rhoe, Rnd3
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P43212
Unit cell:
a: 59.454Å b: 59.454Å c: 233.024Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Function and regulation of Rnd proteins.
Chardin P. (2006)
2 more

3 mentions without citation

Mechanism of multi-site phosphorylation from a ROCK-I:RhoE complex structure.
Komander et al. (2008)
2 more