1gwp Citations

Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein.

Nat Struct Biol 9 537-43 (2002)
Cited: 158 times
EuropePMC logo PMID: 12032547

Abstract

The capsid protein (CA) of the mature human immunodeficiency virus (HIV) contains an N-terminal beta-hairpin that is essential for formation of the capsid core particle. CA is generated by proteolytic cleavage of the Gag precursor polyprotein during viral maturation. We have determined the NMR structure of a 283-residue N-terminal fragment of immature HIV-1 Gag (Gag(283)), which includes the intact matrix (MA) and N-terminal capsid (CA(N)) domains. The beta-hairpin is unfolded in Gag(283), consistent with the proposal that hairpin formation occurs subsequent to proteolytic cleavage of Gag, triggering capsid assembly. Comparison of the immature and mature CA(N) structures reveals that beta-hairpin formation induces a approximately 2 A displacement of helix 6 and a concomitant displacement of the cyclophylin-A (CypA)-binding loop, suggesting a possible allosteric mechanism for CypA-mediated destabilization of the capsid particle during infectivity.

Reviews - 1gwp mentioned but not cited (6)

  1. TRIM5 structure, HIV-1 capsid recognition, and innate immune signaling. Grütter MG, Luban J. Curr Opin Virol 2 142-150 (2012)
  2. Viral precursor polyproteins: keys of regulation from replication to maturation. Yost SA, Marcotrigiano J. Curr Opin Virol 3 137-142 (2013)
  3. Molecular Architecture of the Retroviral Capsid. Perilla JR, Gronenborn AM. Trends Biochem Sci 41 410-420 (2016)
  4. HIV-1 gag: an emerging target for antiretroviral therapy. Tedbury PR, Freed EO. Curr Top Microbiol Immunol 389 171-201 (2015)
  5. Computational Methods in Immunology and Vaccinology: Design and Development of Antibodies and Immunogens. Guarra F, Colombo G. J Chem Theory Comput 19 5315-5333 (2023)
  6. Inhibitors of peptidyl proline isomerases as antivirals in hepatitis C and other viruses. Striker R, Mehle A. PLoS Pathog 10 e1004428 (2014)

Articles - 1gwp mentioned but not cited (19)



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  2. HIV-1 assembly, release and maturation. Freed EO. Nat Rev Microbiol 13 484-496 (2015)
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  5. Assembly and architecture of HIV. Ganser-Pornillos BK, Yeager M, Pornillos O. Adv Exp Med Biol 726 441-465 (2012)
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  7. HIV-1 Maturation: Lessons Learned from Inhibitors. Kleinpeter AB, Freed EO. Viruses 12 E940 (2020)
  8. New insights into HTLV-1 particle structure, assembly, and Gag-Gag interactions in living cells. Fogarty KH, Zhang W, Grigsby IF, Johnson JL, Chen Y, Mueller JD, Mansky LM. Viruses 3 770-793 (2011)
  9. HIV-1 Capsid Core: A Bullet to the Heart of the Target Cell. Toccafondi E, Lener D, Negroni M. Front Microbiol 12 652486 (2021)
  10. The Interplay between HIV-1 Gag Binding to the Plasma Membrane and Env Incorporation. Murphy RE, Saad JS. Viruses 12 E548 (2020)
  11. Structural Analysis of Retrovirus Assembly and Maturation. Krebs AS, Mendonça LM, Zhang P. Viruses 14 54 (2021)
  12. Understanding the process of envelope glycoprotein incorporation into virions in simian and feline immunodeficiency viruses. Affranchino JL, González SA. Viruses 6 264-283 (2014)
  13. Interplay between Host tRNAs and HIV-1: A Structural Perspective. Zhang J. Viruses 13 1819 (2021)
  14. NMR Studies of Retroviral Genome Packaging. Boyd PS, Brown JB, Brown JD, Catazaro J, Chaudry I, Ding P, Dong X, Marchant J, O'Hern CT, Singh K, Swanson C, Summers MF, Yasin S. Viruses 12 E1115 (2020)
  15. Condensation Goes Viral: A Polymer Physics Perspective. Alston JJ, Soranno A. J Mol Biol 435 167988 (2023)
  16. Stephan Oroszlan and the Proteolytic Processing of Retroviral Proteins: Following A Pro. Swanstrom R, Sundquist WI. Viruses 13 2218 (2021)
  17. HIV-1 RNA genome packaging: it's G-rated. Duchon A, Hu W-S. mBio 15 e0086123 (2024)

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