› 1h3j

X-ray diffraction
2Å resolution

STRUCTURE OF RECOMBINANT COPRINUS CINEREUS PEROXIDASE DETERMINED TO 2.0 A

Released:
DOI: 10.2210/pdb1h3j/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1h3j coloured by chain, front view.
PDB entry 1h3j coloured by chain, side view.
PDB entry 1h3j coloured by chain, top view.
Source organism: Coprinopsis cinerea
Primary publication:
Impact of the physical and chemical environment on the molecular structure of Coprinus cinereus peroxidase.
Houborg K, Harris P, Petersen J, Rowland P, Poulsen JC, Schneider P, Vind J, Larsen S
Acta Crystallogr D Biol Crystallogr 59 989-96 (2003)
PMID: 12777760

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151201 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Peroxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 35.52 KDa
Source organism: Coprinopsis cinerea
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P28314 (Residues: 22-363; Coverage: 100%)
Gene name: CIP1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand CA 4 x CA
Ligand BMA 2 x BMA
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 127.18Å b: 75.53Å c: 76.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.256
Expression system: Aspergillus oryzae

Quick links

Citations

8 citation in other articles

Solvated docking: introducing water into the modelling of biomolecular complexes.
van Dijk et al. (2006)
7 more

2 mentions without citation

Mutagenic Organized Recombination Process by Homologous IN vivo Grouping (MORPHING) for directed enzyme evolution.
Gonzalez-Perez et al. (2014)
1 more