› 1h8x

X-ray diffraction
2Å resolution

Domain-swapped Dimer of a Human Pancreatic Ribonuclease Variant

Released:
DOI: 10.2210/pdb1h8x/pdb
PDB entry 1h8x coloured by chain, front view.
PDB entry 1h8x coloured by chain, side view.
PDB entry 1h8x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of an engineered domain-swapped ribonuclease dimer and its implications for the evolution of proteins toward oligomerization.
Canals A, Pous J, Guasch A, Benito A, Ribó M, Vilanova M, Coll M
Structure 9 967-76 (2001)
PMID: 11591351

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139881 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07998 (Residues: 29-156; Coverage: 100%)
Gene names: RIB1, RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 50.61Å b: 61.43Å c: 75.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
6 more

6 mentions without citation

Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.
Gotte et al. (2019)
5 more