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X-ray structure of full-length annexin 1 and implications for membrane aggregation.

Rosengarth A, Gerke V, Luecke H
J Mol Biol 306 489-98 (2001)
Cited: 94 times
EuropePMC logo PMID: 11178908

Abstract

Annexins comprise a multigene family of Ca2+ and phospholipid- binding proteins. They consist of a conserved C-terminal or core domain that confers Ca2+-dependent phospholipid binding and an N-terminal domain that is variable in sequence and length and responsible for the specific properties of each annexin. Crystal structures of various annexin core domains have revealed a high degree of similarity. From these and other studies it is evident that the core domain harbors the calcium-binding sites that interact with the phospholipid headgroups. However, no structure has been reported of an annexin with a complete N-terminal domain. We have now solved the crystal structure of such a full-length annexin, annexin 1. Annexin 1 is active in membrane aggregation and its refined 1.8 A structure shows an alpha-helical N-terminal domain connected to the core domain by a flexible linker. It is surprising that the two alpha-helices present in the N-terminal domain of 41 residues interact intimately with the core domain, with the amphipathic helix 2-12 of the N-terminal domain replacing helix D of repeat III of the core. In turn, helix D is unwound into a flap now partially covering the N-terminal helix. Implications for membrane aggregation will be discussed and a model of aggregation based on the structure will be presented.

Articles - 1hm6 mentioned but not cited (14)

  1. Annexin-phospholipid interactions. Functional implications. Lizarbe MA, Barrasa JI, Olmo N, Gavilanes F, Turnay J. Int J Mol Sci 14 2652-2683 (2013)
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Reviews citing this publication (25)

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Articles citing this publication (55)

  1. Annexin A1: a central player in the anti-inflammatory and neuroprotective role of microglia. McArthur S, McArthur S, Cristante E, Paterno M, Christian H, Roncaroli F, Gillies GE, Solito E. J Immunol 185 6317-6328 (2010)
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Related citations provided by authors (1)

  1. Crystallization and Preliminary X-ray Analysis of Full-length Annexin I Comprising the Core and N-terminal Domain.. Rosengarth A, Luecke H Acta Crystallogr. D Biol. Crystallogr. 56 1459-1461 (2000)

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