› 1hp0

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF AN INOSINE-ADENOSINE-GUANOSINE-PREFERRING NUCLEOSIDE HYDROLASE FROM TRYPANOSOMA VIVAX IN COMPLEX WITH THE SUBSTRATE ANALOGUE 3-DEAZA-ADENOSINE

Released:
DOI: 10.2210/pdb1hp0/pdb
PDB entry 1hp0 coloured by chain, front view.
PDB entry 1hp0 coloured by chain, side view.
PDB entry 1hp0 coloured by chain, top view.
Source organism: Trypanosoma vivax
Primary publication:
Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax.
Versées W, Decanniere K, Pellé R, Depoorter J, Brosens E, Parkin DW, Steyaert J
J Mol Biol 307 1363-79 (2001)
PMID: 11292348

Function and Biology Details

Reaction catalysed: 
A purine nucleoside + H(2)O = D-ribose + a purine base
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine/uridine-preferring nucleoside hydrolase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 37.72 KDa
Source organism: Trypanosoma vivax
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GPQ4 (Residues: 2-327; Coverage: 100%)
Sequence domains: Inosine-uridine preferring nucleoside hydrolase
Structure domains: Ribonucleoside hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand AD3 2 x AD3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21
Unit cell:
a: 51.67Å b: 74.29Å c: 81.51Å
α: 90° β: 103.54° γ: 90°
R-values:
R R work R free
0.191 0.191 0.254
Expression system: Escherichia coli

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Citations

5 review citations

Lessons learned from the genome analysis of ralstonia solanacearum.
Genin et al. (2004)
4 more

7 mentions without citation

Variability in docking success rates due to dataset preparation.
Corbeil et al. (2012)
6 more