› 1hr0

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF INITIATION FACTOR IF1 BOUND TO THE 30S RIBOSOMAL SUBUNIT

Released:
DOI: 10.2210/pdb1hr0/pdb
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Source organisms:
Primary publication:
Crystal structure of an initiation factor bound to the 30S ribosomal subunit.
Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Hartsch T, Wimberly BT, Ramakrishnan V
Science 291 498-501 (2001)
PMID: 11228145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 23-mer (preferred)
PDBe Complex ID:
PDB-CPX-289804 (preferred)
Entry contents:
21 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (23 distinct):
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 256 amino acids
Theoretical weight: 29.32 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80371 (Residues: 1-256; Coverage: 100%)
Gene names: TTHA0861, rps2, rpsB
Sequence domains: Ribosomal protein S2
Structure domains:
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 239 amino acids
Theoretical weight: 26.75 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80372 (Residues: 1-239; Coverage: 100%)
Gene names: TTHA1686, rps3, rpsC
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein bTHX Chain: V
Translation initiation factor IF-1 Chain: W
16S ribosomal RNA Chain: A
Messenger RNA Chain: X

Ligands and Environments

2 bound ligands:
Ligand MG 65 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 399.581Å b: 399.581Å c: 176.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.261
Expression system: Escherichia coli

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Citations

63 review citations

Ribosome structure and the mechanism of translation.
Ramakrishnan V. (2002)
62 more

23 mentions without citation

Initiation of protein synthesis in bacteria.
Laursen et al. (2005)
22 more

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