› 1hxp

X-ray diffraction
1.8Å resolution

NUCLEOTIDE TRANSFERASE

Released:
DOI: 10.2210/pdb1hxp/pdb
PDB entry 1hxp coloured by chain, front view.
PDB entry 1hxp coloured by chain, side view.
PDB entry 1hxp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Three-dimensional structure of galactose-1-phosphate uridylyltransferase from Escherichia coli at 1.8 A resolution.
Wedekind JE, Frey PA, Rayment I
Biochemistry 34 11049-61 (1995)
PMID: 7669762

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose-1-phosphate uridylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 39.7 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09148 (Residues: 1-348; Coverage: 100%)
Gene names: JW0741, b0758, galB, galT
Sequence domains:
Structure domains: HIT-like

Ligands and Environments

5 bound ligands:
Ligand ZN 2 x ZN
Ligand FE 2 x FE
Ligand BME 4 x BME
Ligand U5P 1 x U5P
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 58.6Å b: 217.2Å c: 69.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 not available not available
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Structural and functional studies of UDP-glucuronosyltransferases.
Radominska-Pandya et al. (1999)
10 more

4 mentions without citation

Alteration of oligomeric state and domain architecture is essential for functional transformation between transferase and hydrolase with the same scaffold.
Koike et al. (2009)
3 more