› 1hy1

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF WILD TYPE DUCK DELTA 2 CRYSTALLIN (EYE LENS PROTEIN)

Released:
DOI: 10.2210/pdb1hy1/pdb
PDB entry 1hy1 coloured by chain, front view.
PDB entry 1hy1 coloured by chain, side view.
PDB entry 1hy1 coloured by chain, top view.
Source organism: Anas platyrhynchos
Primary publication:
Structural studies of duck delta 1 and delta 2 crystallin suggest conformational changes occur during catalysis.
Sampaleanu LM, Vallée F, Slingsby C, Howell PL
Biochemistry 40 2732-42 (2001)
PMID: 11258884

Function and Biology Details

Reaction catalysed: 
2-(N(omega)-L-arginino)succinate = fumarate + L-arginine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Argininosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 468 amino acids
Theoretical weight: 51.77 KDa
Source organism: Anas platyrhynchos
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P24058 (Residues: 1-468; Coverage: 100%)
Gene name: ASL2
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 93.481Å b: 99.052Å c: 107.177Å
α: 90° β: 102.08° γ: 90°
R-values:
R R work R free
0.236 0.208 0.263
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Moonlighting proteins--an update.
Jeffery CJ. (2009)
2 more

4 mentions without citation

Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis.
Sampaleanu et al. (2004)
3 more