› 1i94

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3

Released:
DOI: 10.2210/pdb1i94/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1i94 coloured by chain, front view.
PDB entry 1i94 coloured by chain, side view.
PDB entry 1i94 coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3.
Pioletti M, Schlünzen F, Harms J, Zarivach R, Glühmann M, Avila H, Bashan A, Bartels H, Auerbach T, Jacobi C, Hartsch T, Yonath A, Franceschi F
EMBO J 20 1829-39 (2001)
PMID: 11296217

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-289810 (preferred)
Entry contents:
20 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 255 amino acids
Theoretical weight: 29.19 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80371 (Residues: 2-256; Coverage: 100%)
Gene names: TTHA0861, rps2, rpsB
Sequence domains: Ribosomal protein S2
Structure domains:
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.62 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: P80372 (Residues: 2-239; Coverage: 100%)
Gene names: TTHA1686, rps3, rpsC
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein bTHX Chain: U
16S ribosomal RNA Chain: A

Ligands and Environments

3 bound ligands:
Ligand MG 75 x MG
Ligand WO2 14 x WO2
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID, ESRF BEAMLINE ID14-2, MPG/DESY, HAMBURG BEAMLINE BW6, EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P41212
Unit cell:
a: 406.3Å b: 406.3Å c: 173.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.203 0.245

Quick links

Citations

76 review citations

Ribosome structure and the mechanism of translation.
Ramakrishnan V. (2002)
75 more

10 mentions without citation

The use of polyoxometalates in protein crystallography - An attempt to widen a well-known bottleneck.
Bijelic et al. (2015)
9 more