1i9s Citations

Structure and mechanism of the RNA triphosphatase component of mammalian mRNA capping enzyme.

EMBO J 20 2575-86 (2001)
Cited: 61 times
EuropePMC logo PMID: 11350947

Abstract

The 5' capping of mammalian pre-mRNAs is initiated by RNA triphosphatase, a member of the cysteine phosphatase superfamily. Here we report the 1.65 A crystal structure of mouse RNA triphosphatase, which reveals a deep, positively charged active site pocket that can fit a 5' triphosphate end. Structural, biochemical and mutational results show that despite sharing an HCxxxxxR(S/T) motif, a phosphoenzyme intermediate and a core alpha/beta-fold with other cysteine phosphatases, the mechanism of phosphoanhydride cleavage by mammalian capping enzyme differs from that used by protein phosphatases to hydrolyze phosphomonoesters. The most significant difference is the absence of a carboxylate general acid catalyst in RNA triphosphatase. Residues conserved uniquely among the RNA phosphatase subfamily are important for function in cap formation and are likely to play a role in substrate recognition.

Reviews - 1i9s mentioned but not cited (1)

  1. Enzymology of RNA cap synthesis. Ghosh A, Lima CD. Wiley Interdiscip Rev RNA 1 152-172 (2010)

Articles - 1i9s mentioned but not cited (3)



Reviews citing this publication (8)

  1. mRNA capping: biological functions and applications. Ramanathan A, Robb GB, Chan SH. Nucleic Acids Res 44 7511-7526 (2016)
  2. Conventional and unconventional mechanisms for capping viral mRNA. Decroly E, Ferron F, Lescar J, Canard B. Nat Rev Microbiol 10 51-65 (2011)
  3. What messenger RNA capping tells us about eukaryotic evolution. Shuman S. Nat Rev Mol Cell Biol 3 619-625 (2002)
  4. Chemical approaches to detect and analyze protein sulfenic acids. Furdui CM, Poole LB. Mass Spectrom Rev 33 126-146 (2014)
  5. The viral RNA capping machinery as a target for antiviral drugs. Ferron F, Decroly E, Selisko B, Canard B. Antiviral Res 96 21-31 (2012)
  6. Function and Structural Organization of the Replication Protein of Bamboo mosaic virus. Meng M, Lee CC. Front Microbiol 8 522 (2017)
  7. Enzymatic Assays to Explore Viral mRNA Capping Machinery. Kasprzyk R, Jemielity J. Chembiochem 22 3236-3253 (2021)
  8. [Capping strategies in RNA viruses]. Bouvet M, Ferron F, Imbert I, Gluais L, Selisko B, Coutard B, Canard B, Decroly E. Med Sci (Paris) 28 423-429 (2012)

Articles citing this publication (49)