› 1ic8

X-ray diffraction
2.6Å resolution

HEPATOCYTE NUCLEAR FACTOR 1A BOUND TO DNA : MODY3 GENE PRODUCT

Released:
DOI: 10.2210/pdb1ic8/pdb
PDB entry 1ic8 coloured by chain, front view.
PDB entry 1ic8 coloured by chain, side view.
PDB entry 1ic8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Diabetes mutations delineate an atypical POU domain in HNF-1alpha.
Chi YI, Frantz JD, Oh BC, Hansen L, Dhe-Paganon S, Shoelson SE
Mol Cell 10 1129-37 (2002)
PMID: 12453420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113950 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Hepatocyte nuclear factor 1-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20823 (Residues: 85-278; Coverage: 31%)
Gene names: HNF1A, TCF1
Sequence domains: Hepatocyte nuclear factor 1 (HNF-1), N terminus
Structure domains:
5'-D(*CP*TP*TP*GP*GP*TP*TP*AP*AP*TP*AP*AP*TP*TP*CP*AP*CP*CP*AP*GP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 21 nucleotides
Theoretical weight: 6.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*TP*CP*TP*GP*GP*TP*GP*AP*AP*TP*TP*AP*TP*TP*AP*AP*CP*CP*AP*AP*G)-3' Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.46 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41
Unit cell:
a: 50.389Å b: 50.389Å c: 207.272Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.254 0.312
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Homeodomain proteins: an update.
Bürglin et al. (2016)
8 more

21 mentions without citation

Homeodomain revisited: a lesson from disease-causing mutations.
Chi YI. (2005)
20 more