› 1iol

X-ray diffraction
2.3Å resolution

ESTROGENIC 17-BETA HYDROXYSTEROID DEHYDROGENASE COMPLEXED 17-BETA-ESTRADIOL

Released:
DOI: 10.2210/pdb1iol/pdb
PDB entry 1iol coloured by chain, front view.
PDB entry 1iol coloured by chain, side view.
PDB entry 1iol coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human estrogenic 17 beta-hydroxysteroid dehydrogenase complexed with 17 beta-estradiol.
Azzi A, Rehse PH, Zhu DW, Campbell RL, Labrie F, Lin SX
Nat Struct Biol 3 665-8 (1996)
PMID: 8756321

Function and Biology Details

Reactions catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17-beta-hydroxysteroid dehydrogenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 34.9 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P14061 (Residues: 2-328; Coverage: 100%)
Gene names: E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17B1, SDR28C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand EST 1 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 123.66Å b: 45.19Å c: 61.3Å
α: 90° β: 99.1° γ: 90°
R-values:
R R work R free
0.196 0.196 0.257

Quick links

Citations

11 review citations

Molecular endocrinology of hydroxysteroid dehydrogenases.
Penning TM. (1997)
10 more

16 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
15 more