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Stabilization of hen egg white lysozyme by a cavity-filling mutation.

Ohmura T, Ueda T, Ootsuka K, Saito M, Imoto T
Protein Sci 10 313-20 (2001)
Related entries: 1ioq, 1ios, 1iot

Cited: 23 times
EuropePMC logo PMID: 11266617

Abstract

Stabilization of a protein using cavity-filling strategy has hardly been successful because of unfavorable van der Waals contacts. We succeeded in stabilizing lysozymes by cavity-filling mutations. The mutations were checked by a simple energy minimization in advance. It was shown clearly that the sum of free energy change caused by the hydrophobicity and the cavity size was correlated very well with protein stability. We also considered the aromatic-aromatic interaction. It is reconfirmed that the cavity-filling mutation in a hydrophobic core is a very useful method to stabilize a protein when the mutation candidate is selected carefully.

Articles citing this publication (23)

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  11. Copper(II) directs formation of toxic amorphous aggregates resulting in inhibition of hen egg white lysozyme fibrillation under alkaline salt-mediated conditions. Ghosh S, Pandey NK, Banerjee P, Chaudhury K, Nagy NV, Dasgupta S. J Biomol Struct Dyn 33 991-1007 (2015)
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  15. Comparing Residue Clusters from Thermophilic and Mesophilic Enzymes Reveals Adaptive Mechanisms. Sammond DW, Kastelowitz N, Himmel ME, Yin H, Crowley MF, Bomble YJ. PLoS One 11 e0145848 (2016)
  16. Stabilization of a metabolic enzyme by library selection in Thermus thermophilus. Schwab T, Sterner R. Chembiochem 12 1581-1588 (2011)
  17. High-Performance Analysis of Biomolecular Containers to Measure Small-Molecule Transport, Transbilayer Lipid Diffusion, and Protein Cavities. Bryer AJ, Hadden-Perilla JA, Stone JE, Perilla JR. J Chem Inf Model 59 4328-4338 (2019)
  18. Crystal structures of K33 mutant hen lysozymes with enhanced activities. Goto T, Ohkuri T, Shioi S, Abe Y, Imoto T, Ueda T. J Biochem 144 619-623 (2008)
  19. Crystal structures of highly simplified BPTIs provide insights into hydration-driven increase of unfolding enthalpy. Islam MM, Yohda M, Kidokoro SI, Kuroda Y. Sci Rep 7 41205 (2017)
  20. Redesigning protein cavities as a strategy for increasing affinity in protein-protein interaction: interferon- γ receptor 1 as a model. Černý J, Biedermannová L, Mikulecký P, Zahradník J, Charnavets T, Šebo P, Schneider B. Biomed Res Int 2015 716945 (2015)
  21. Stability and solubility engineering of the EphB4 tyrosine kinase catalytic domain using a rationally designed synthetic library. Overman RC, Green I, Truman CM, Read JA, Embrey KJ, McAlister MS, Attwood TK. Protein Eng Des Sel 26 695-704 (2013)
  22. Inside Out Computational Redesign of Cavities for Improving Thermostability and Catalytic Activity of Rhizomucor Miehei Lipase. Zhang Z, Long M, Zheng N, Lü X, Zhu C, Osire T, Xia X. Appl Environ Microbiol 89 e0217222 (2023)
  23. Protein Engineering of the Soluble Metal-dependent Formate Dehydrogenase from Escherichia coli. Fuji R, Umezawa K, Mizuguchi M, Ihara M. Anal Sci 37 733-739 (2021)


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