› 1isb

X-ray diffraction
1.85Å resolution

STRUCTURE-FUNCTION IN E. COLI IRON SUPEROXIDE DISMUTASE: COMPARISONS WITH THE MANGANESE ENZYME FROM T. THERMOPHILUS

Released:
DOI: 10.2210/pdb1isb/pdb
PDB entry 1isb coloured by chain, front view.
PDB entry 1isb coloured by chain, side view.
PDB entry 1isb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure-function in Escherichia coli iron superoxide dismutase: comparisons with the manganese enzyme from Thermus thermophilus.
Lah MS, Dixon MM, Pattridge KA, Stallings WC, Fee JA, Ludwig ML
Biochemistry 34 1646-60 (1995)
PMID: 7849024

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-533302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Fe] Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 21.15 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0AGD3 (Residues: 2-193; Coverage: 100%)
Gene names: JW1648, b1656, sodB
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand FE 2 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 81.62Å b: 75.28Å c: 71.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

The 2-His-1-carboxylate facial triad--an emerging structural motif in mononuclear non-heme iron(II) enzymes.
Hegg et al. (1997)
8 more

7 mentions without citation

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
6 more