› 1isf

X-ray diffraction
2.5Å resolution

Crystal Structure Analysis of BST-1/CD157

Released:
DOI: 10.2210/pdb1isf/pdb
Model geometry
Fit model/data
PDB entry 1isf coloured by chain, front view.
PDB entry 1isf coloured by chain, side view.
PDB entry 1isf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase activities.
Yamamoto-Katayama S, Ariyoshi M, Ishihara K, Hirano T, Jingami H, Morikawa K
J Mol Biol 316 711-23 (2002)
PMID: 11866528

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.17 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q10588 (Residues: 33-297; Coverage: 93%)
Gene name: BST1
Sequence domains: ADP-ribosyl cyclase
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P212121
Unit cell:
a: 58.721Å b: 112.612Å c: 130.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.25
Expression system: Trichoplusia ni

Quick links

Citations

16 review citations

Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology.
Malavasi et al. (2008)
15 more

2 mentions without citation

Benchmark Sets for Binding Hot Spot Identification in Fragment-Based Ligand Discovery.
Wakefield et al. (2020)
1 more