› › Function and Biology

Crystal Structure Of Archaeosine tRNA-Guanine Transglycosylase Complexed With lambda-form tRNA(Val)

Source organism: Pyrococcus horikoshii
Biochemical function: metal ion binding
Biological process: queuosine biosynthetic process
Cellular component: cytoplasm

Quick links

EC 2.4.2.48: tRNA-guanine(15) transglycosylase

Reaction catalysed:
Guanine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanine(15) in tRNA + guanine
Systematic name:
tRNA-guanine(15):7-cyano-7-carbaguanine tRNA-D-ribosyltransferase
Alternative Name(s):
  • TGT
  • Transfer ribonucleate glycosyltransferase
  • Transfer ribonucleic acid guanine(15) transglycosylase
  • tRNA guanine(15) transglycosidase
  • tRNA transglycosylase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF01472
Domain description: PUA domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of Pfam domain PF01472 (PUA domain) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].

PF14810
Domain description: Patch-forming domain C2 of tRNA-guanine transglycosylase
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of Pfam domain PF14810 (Patch-forming domain C2 of tRNA-guanine transglycosylase) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].

PF14809
Domain description: C1 domain of tRNA-guanine transglycosylase dimerisation
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of Pfam domain PF14809 (C1 domain of tRNA-guanine transglycosylase dimerisation) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].

PF01702
Domain description: Queuine tRNA-ribosyltransferase
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of Pfam domain PF01702 (Queuine tRNA-ribosyltransferase) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].

Rfam RNA family (Rfam)
RF00005
Domain description: tRNA
Occurring in:
  1. tRNA(Val)
The deposited structure of PDB entry 1j2b contains 2 copies of Rfam domain RF00005 (tRNA) in tRNA(Val). Showing 1 copy in chain A [auth C].

InterPro InterPro annotations
IPR002478
Domain description: PUA domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR004804
Domain description: tRNA-guanine(15) transglycosylase
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR002616
Domain description: tRNA-guanine(15) transglycosylase-like
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR036511
Domain description: Queuine tRNA-ribosyltransferase-like
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR015947
Domain description: PUA-like superfamily
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR036974
Domain description: PUA domain superfamily
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR004521
Domain description: Uncharacterised domain CHP00451
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR029402
Domain description: tRNA-guanine transglycosylase, patch-forming domain C2
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR038250
Domain description: TGT, patch-forming C2 domain superfamily
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR038370
Domain description: ArcTGT, C1 domain superfamily
Occurring in:
  1. tRNA-guanine(15) transglycosylase
IPR032729
Domain description: tRNA-guanine(15) transglycosylase, C1 domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase

Structure domains

CATH CATH domains
2.30.130.10
Class: Mainly Beta
Architecture: Roll
Topology: Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4
Homology: PUA domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of CATH domain 2.30.130.10 (Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 4) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].
3.10.450.90
Class: Alpha Beta
Architecture: Roll
Topology: Nuclear Transport Factor 2; Chain: A,
Homology: ArcTGT, C2 domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of CATH domain 3.10.450.90 (Nuclear Transport Factor 2; Chain: A,) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].
3.90.1020.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 2
Homology: ArcTGT, C1 domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of CATH domain 3.90.1020.10 (Archaeosine Trna-guanine Transglycosylase; Chain: A, domain 2) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].
3.20.20.105
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Queuine tRNA-ribosyltransferase-like
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of CATH domain 3.20.20.105 (TIM Barrel) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].
SCOP SCOP annotations
88698
Class: All beta proteins
Fold: PUA domain-like
Superfamily: PUA domain-like
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of SCOP domain 88698 (PUA domain) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].
88803
Class: Alpha and beta proteins (a+b)
Fold: Cystatin-like
Superfamily: Pre-PUA domain
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of SCOP domain 88803 (Archaeosine tRNA-guanine transglycosylase, C2 domain) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].
51714
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: tRNA-guanine transglycosylase
Occurring in:
  1. tRNA-guanine(15) transglycosylase
The deposited structure of PDB entry 1j2b contains 2 copies of SCOP domain 51714 (tRNA-guanine transglycosylase) in tRNA-guanine(15) transglycosylase. Showing 1 copy in chain C [auth A].

Quick links