› 1jmt

X-ray diffraction
2.2Å resolution

X-ray Structure of a Core U2AF65/U2AF35 Heterodimer

Released:
DOI: 10.2210/pdb1jmt/pdb
PDB entry 1jmt coloured by chain, front view.
PDB entry 1jmt coloured by chain, side view.
PDB entry 1jmt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A novel peptide recognition mode revealed by the X-ray structure of a core U2AF35/U2AF65 heterodimer.
Kielkopf CL, Rodionova NA, Green MR, Burley SK
Cell 106 595-605 (2001)
PMID: 11551507

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150719 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor U2AF 35 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 104 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q01081 (Residues: 43-146; Coverage: 43%)
  • Best match: Q01081-4 (Residues: 1-73)
Gene names: FP793, U2AF1, U2AF35, U2AFBP
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Splicing factor U2AF 65 kDa subunit Chain: B
Molecule details ›
Chain: B
Length: 28 amino acids
Theoretical weight: 3.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P26368 (Residues: 85-112; Coverage: 6%)
Gene names: U2AF2, U2AF65

Ligands and Environments

1 bound ligand:
Ligand HEZ 2 x HEZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25, CHESS BEAMLINE F2
Spacegroup: P222
Unit cell:
a: 41.52Å b: 49.74Å c: 92.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.238
Expression system: Escherichia coli BL21

Quick links

Citations

22 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
21 more

17 mentions without citation

U2AF homology motifs: protein recognition in the RRM world.
Kielkopf et al. (2004)
16 more