› 1jr0

X-ray diffraction
1.3Å resolution

CHOLERA TOXIN B-PENTAMER WITH LIGAND BMSC-0011

Released:
DOI: 10.2210/pdb1jr0/pdb
PDB entry 1jr0 coloured by chain, front view.
PDB entry 1jr0 coloured by chain, side view.
PDB entry 1jr0 coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Anchor-based design of improved cholera toxin and E. coli heat-labile enterotoxin receptor binding antagonists that display multiple binding modes.
Pickens JC, Merritt EA, Ahn M, Verlinde CL, Hol WG, Fan E
Chem Biol 9 215-24 (2002)
PMID: 11880036

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo pentamer (preferred)
homo decamer
Assembly name:
PDBe Complex ID:
PDB-CPX-134384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholera enterotoxin subunit B Chains: D, E, F, G, H
Molecule details ›
Chains: D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.62 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P01556 (Residues: 22-124; Coverage: 100%)
Gene names: VC_1456, ctxB, toxB
Sequence domains: Heat-labile enterotoxin beta chain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand A24 5 x A24
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 102.098Å b: 66.064Å c: 78.565Å
α: 90° β: 105.74° γ: 90°
R-values:
R R work R free
0.131 not available 0.183
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Design and creativity in synthesis of multivalent neoglycoconjugates.
Chabre et al. (2010)
5 more

1 mention without citation

Expression, purification and crystallization of CTB-MPR, a candidate mucosal vaccine component against HIV-1.
Lee et al. (2014)