› 1ju9

X-ray diffraction
2Å resolution

HORSE LIVER ALCOHOL DEHYDROGENASE VAL292SER MUTANT

Released:
DOI: 10.2210/pdb1ju9/pdb
PDB entry 1ju9 coloured by chain, front view.
PDB entry 1ju9 coloured by chain, side view.
PDB entry 1ju9 coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Contributions of valine-292 in the nicotinamide binding site of liver alcohol dehydrogenase and dynamics to catalysis.
Rubach JK, Ramaswamy S, Plapp BV
Biochemistry 40 12686-94 (2001)
PMID: 11601993

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.84 KDa
Source organism: Equus caballus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 46.271Å b: 180.398Å c: 46.176Å
α: 90° β: 106.65° γ: 90°
R-values:
R R work R free
0.196 0.196 0.232
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Multidimensional tunneling, recrossing, and the transmission coefficient for enzymatic reactions.
Pu et al. (2006)
3 more

1 mention without citation

Effects of cavities at the nicotinamide binding site of liver alcohol dehydrogenase on structure, dynamics and catalysis.
Yahashiri et al. (2014)