› 1jv5

X-ray diffraction
2.2Å resolution

Anti-blood group A Fv

Released:
DOI: 10.2210/pdb1jv5/pdb
PDB entry 1jv5 coloured by chain, front view.
PDB entry 1jv5 coloured by chain, side view.
PDB entry 1jv5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of an anti-blood group A Fv and improvement of its binding affinity without loss of specificity.
Thomas R, Patenaude SI, MacKenzie CR, To R, Hirama T, Young NM, Evans SV
J Biol Chem 277 2059-64 (2002)
PMID: 11679577

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208451 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin kappa variable 1-33 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 11.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01594 (Residues: 23-117; Coverage: 100%)
Gene name: IGKV1-33
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig chain heavy chain precursor V region Chain: B
Molecule details ›
Chain: B
Length: 117 amino acids
Theoretical weight: 12.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 51.71Å b: 57.96Å c: 83.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.183 0.223
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The nature of target-unrelated peptides recovered in the screening of phage-displayed random peptide libraries with antibodies.
Menendez et al. (2005)
3 more

4 mentions without citation

Crystal structure of the engineered neutralizing antibody M18 complexed to domain 4 of the anthrax protective antigen.
Leysath et al. (2009)
3 more