› 1jvz

X-ray diffraction
2.6Å resolution

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid

Released:
DOI: 10.2210/pdb1jvz/pdb
PDB entry 1jvz coloured by chain, front view.
PDB entry 1jvz coloured by chain, side view.
PDB entry 1jvz coloured by chain, top view.
Source organism: Brevundimonas diminuta
Primary publication:
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity.
Kim Y, Hol WG
Chem Biol 8 1253-64 (2001)
PMID: 11755403

Function and Biology Details

Reaction catalysed: 
(7R)-7-(4-carboxybutanamido)cephalosporanate + H(2)O = (7R)-7-aminocephalosporanate + glutarate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192214 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Glutaryl-7-aminocephalosporanic-acid acylase subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.47 KDa
Source organism: Brevundimonas diminuta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L5D6 (Residues: 30-187; Coverage: 23%)
Structure domains: Penicillin Amidohydrolase, domain 1
Glutaryl-7-aminocephalosporanic-acid acylase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 520 amino acids
Theoretical weight: 58.69 KDa
Source organism: Brevundimonas diminuta
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L5D6 (Residues: 199-718; Coverage: 75%)
Sequence domains: Penicillin amidase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CEN 1 x CEN
1 modified residue:
Ligand MSE 13 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 74.14Å b: 74.14Å c: 379.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.214
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nonribosomal peptide synthetase biosynthetic clusters of ESKAPE pathogens.
Gulick AM. (2017)
3 more

7 mentions without citation

Evolution of an acylase active on cephalosporin C.
Pollegioni et al. (2005)
6 more