› 1k0t

Solution NMR

NMR SOLUTION STRUCTURE OF UNBOUND, OXIDIZED PHOTOSYSTEM I SUBUNIT PSAC, CONTAINING [4FE-4S] CLUSTERS FA AND FB

Released:
DOI: 10.2210/pdb1k0t/pdb
PDB entry 1k0t coloured by chain, ensemble of 30 models, front view.
PDB entry 1k0t coloured by chain, ensemble of 30 models, side view.
PDB entry 1k0t coloured by chain, ensemble of 30 models, top view.
Source organism: Synechococcus sp. PCC 7002
Primary publication:
Solution structure of the unbound, oxidized Photosystem I subunit PsaC, containing [4Fe-4S] clusters F(A) and F(B): a conformational change occurs upon binding to photosystem I.
Antonkine ML, Liu G, Bentrop D, Bryant DA, Bertini I, Luchinat C, Golbeck JH, Stehlik D
J Biol Inorg Chem 7 461-72 (2002)
PMID: 11941504

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-542357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photosystem I iron-sulfur center Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 8.69 KDa
Source organism: Synechococcus sp. PCC 7002
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31087 (Residues: 2-81; Coverage: 99%)
Gene names: SYNPCC7002_A1589, psaC
Sequence domains: 4Fe-4S dicluster domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand SF4 2 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics combined with a simulated annealing algorithm
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Structure of cyanobacterial photosystem I.
Grotjohann et al. (2005)
4 more

1 mention without citation

Assembly of protein subunits within the stromal ridge of photosystem I. Structural changes between unbound and sequentially PS I-bound polypeptides and correlated changes of the magnetic properties of the terminal iron sulfur clusters.
Antonkine et al. (2003)