› 1k2o

X-ray diffraction
1.65Å resolution

Cytochrome P450Cam with Bound BIS(2,2'-BIPYRIDINE)-(5-METHYL-2-2'-BIPYRIDINE)-C2-ADAMANTANE RUTHENIUM (II)

Released:
DOI: 10.2210/pdb1k2o/pdb
PDB entry 1k2o coloured by chain, front view.
PDB entry 1k2o coloured by chain, side view.
PDB entry 1k2o coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Probing the open state of cytochrome P450cam with ruthenium-linker substrates.
Dunn AR, Dmochowski IJ, Bilwes AM, Gray HB, Crane BR
Proc Natl Acad Sci U S A 98 12420-5 (2001)
PMID: 11606730

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-523344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 46.56 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
3 bound ligands:
Ligand CAC 5 x CAC
Ligand RFA 2 x RFA
Ligand RFB 2 x RFB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P1
Unit cell:
a: 63.87Å b: 67.05Å c: 72.52Å
α: 71.16° β: 65.2° γ: 62.31°
R-values:
R R work R free
0.21 0.21 0.226
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Cytochrome P450: what have we learned and what are the future issues?
Guengerich FP. (2004)
6 more

6 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
5 more