› 1k5j

X-ray diffraction
2.3Å resolution

The Crystal Structure of Nucleoplasmin-Core

Released:
DOI: 10.2210/pdb1k5j/pdb
PDB entry 1k5j coloured by chain, front view.
PDB entry 1k5j coloured by chain, side view.
PDB entry 1k5j coloured by chain, top view.
Source organism: Xenopus laevis
Primary publication:
The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly.
Dutta S, Akey IV, Dingwall C, Hartman KL, Laue T, Nolte RT, Head JF, Akey CW
Mol Cell 8 841-53 (2001)
PMID: 11684019

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-529197 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoplasmin Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 124 amino acids
Theoretical weight: 13.75 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05221 (Residues: 1-124; Coverage: 62%)
Sequence domains: Nucleoplasmin/nucleophosmin domain
Structure domains: Nucleoplasmin core domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 116.7Å b: 67.1Å c: 101.7Å
α: 90° β: 123.3° γ: 90°
R-values:
R R work R free
0.251 0.219 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

22 review citations

Histone chaperone networks shaping chromatin function.
Hammond et al. (2017)
21 more

16 mentions without citation

Chaperoning histones during DNA replication and repair.
Ransom et al. (2010)
15 more