› 1kbw

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE SOLUBLE DOMAIN OF ANIA FROM NEISSERIA GONORRHOEAE

Released:
DOI: 10.2210/pdb1kbw/pdb
PDB entry 1kbw coloured by chain, front view.
PDB entry 1kbw coloured by chain, side view.
PDB entry 1kbw coloured by chain, top view.
Source organism: Neisseria gonorrhoeae
Primary publication:
Crystal structure of the soluble domain of the major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria: a new class of copper-containing nitrite reductases.
Boulanger MJ, Murphy ME
J Mol Biol 315 1111-27 (2002)
PMID: 11827480

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 327 amino acids
Theoretical weight: 35.21 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02219 (Residues: 42-364; Coverage: 86%)
Gene name: aniA
Sequence domains: Multicopper oxidase
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 12 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P1
Unit cell:
a: 66.23Å b: 99.55Å c: 103.71Å
α: 83.55° β: 73.7° γ: 73.01°
R-values:
R R work R free
0.211 0.211 0.234
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Copper active sites in biology.
Solomon et al. (2014)
10 more

9 mentions without citation

The surface lipoproteins of gram-negative bacteria: Protectors and foragers in harsh environments.
Cole et al. (2021)
8 more