› 1kcb

X-ray diffraction
1.65Å resolution

Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction

Released:
DOI: 10.2210/pdb1kcb/pdb
PDB entry 1kcb coloured by chain, front view.
PDB entry 1kcb coloured by chain, side view.
PDB entry 1kcb coloured by chain, top view.
Source organism: Achromobacter cycloclastes
Primary publication:
Crystal structure of a NO-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction.
Liu SQ, Chang T, Liu MY, LeGall J, Chang WC, Zhang JP, Liang DC, Chang WR
Biochem Biophys Res Commun 302 568-74 (2003)
PMID: 12615072

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.08 KDa
Source organism: Achromobacter cycloclastes
Expression system: Escherichia coli
UniProt:
  • Canonical: P25006 (Residues: 39-378; Coverage: 100%)
Gene name: nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P213
Unit cell:
a: 98.16Å b: 98.16Å c: 98.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.17 0.204
Expression system: Escherichia coli

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Citations

1 citations in other articles

Peptide Inhibitors Targeting the Neisseria gonorrhoeae Pivotal Anaerobic Respiration Factor AniA.
Sikora et al. (2017)