› 1kgw

X-ray diffraction
2.1Å resolution

THREE DIMENSIONAL STRUCTURE ANALYSIS OF THE R337Q VARIANT OF HUMAN PANCREATIC ALPHA-MYLASE

Released:
DOI: 10.2210/pdb1kgw/pdb
PDB entry 1kgw coloured by chain, front view.
PDB entry 1kgw coloured by chain, side view.
PDB entry 1kgw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Probing the role of the chloride ion in the mechanism of human pancreatic alpha-amylase.
Numao S, Maurus R, Sidhu G, Wang Y, Overall CM, Brayer GD, Withers SG
Biochemistry 41 215-25 (2002)
PMID: 11772019

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 55.9 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P04746 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2A
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 1 x CA
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 52.943Å b: 74.934Å c: 137.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 not available
Expression system: Komagataella pastoris

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Citations

1 review citation

Effect of reducing sodium chloride based on the sensory properties of meat products and the improvement strategies employed: a review.
Kim et al. (2021)
24 other articles