› 1kho

X-ray diffraction
2.4Å resolution

Crystal Structure Analysis of Clostridium perfringens alpha-Toxin Isolated from Avian Strain SWCP

Released:
DOI: 10.2210/pdb1kho/pdb
PDB entry 1kho coloured by chain, front view.
PDB entry 1kho coloured by chain, side view.
PDB entry 1kho coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
The first strain of Clostridium perfringens isolated from an avian source has an alpha-toxin with divergent structural and kinetic properties.
Justin N, Walker N, Bullifent HL, Songer G, Bueschel DM, Jost H, Naylor C, Miller J, Moss DS, Titball RW, Basak AK
Biochemistry 41 6253-62 (2002)
PMID: 12009886

Function and Biology Details

Reaction catalysed: 
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-576856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 42.42 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9RF12 (Residues: 29-398; Coverage: 100%)
Gene names: cpa, plc
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P4122
Unit cell:
a: 117.23Å b: 117.23Å c: 220.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.236
Expression system: Escherichia coli BL21

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Citations

2 review citations

Clostridial toxins.
Popoff et al. (2009)
1 more

8 mentions without citation

Molecular chaperones are nanomachines that catalytically unfold misfolded and alternatively folded proteins.
Mattoo et al. (2014)
7 more