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Comparative analysis of space-grown and earth-grown crystals of an aminoacyl-tRNA synthetase: space-grown crystals are more useful for structural determination.

Ng JD, Sauter C, Lorber B, Kirkland N, Arnez J, Giegé R
Acta Crystallogr D Biol Crystallogr 58 645-52 (2002)
Cited: 13 times
EuropePMC logo PMID: 11914489

Abstract

Protein crystallization under microgravity aims at benefiting from the quasi-absence of convection and sedimentation to favor well ordered crystal nucleation and growth. The dimeric multidomain enzyme aspartyl-tRNA synthetase from Thermus thermophilus has been crystallized within dialysis reactors of the Advanced Protein Crystallization Facility in the laboratory on earth and under microgravity aboard the US Space Shuttle. A strictly comparative crystallographic analysis reveals that the crystals grown in space are superior in every respect to control crystals prepared in otherwise identical conditions on earth. They diffract X-rays more intensely and have a lower mosaicity, facilitating the process of protein structure determination. Indeed, the electron-density map calculated from diffraction data of space-grown crystals contains considerably more detail. The resulting three-dimensional structure model at 2.0 A resolution is more accurate than that produced in parallel using the data originating from earth-grown crystals. The major differences between the structures, including the better defined amino-acid side chains and the higher order of bound water molecules, are emphasized.

Reviews - 1l0w mentioned but not cited (1)

  1. Overview of protein structural and functional folds. Sun PD, Foster CE, Boyington JC. Curr Protoc Protein Sci Chapter 17 Unit 17.1 (2004)

Articles - 1l0w mentioned but not cited (3)

  1. Structural properties of PAS domains from the KCNH potassium channels. Adaixo R, Harley CA, Castro-Rodrigues AF, Morais-Cabral JH. PLoS One 8 e59265 (2013)
  2. LBSL: Case Series and DARS2 Variant Analysis in Early Severe Forms With Unexpected Presentations. Stellingwerff MD, Figuccia S, Bellacchio E, Alvarez K, Castiglioni C, Topaloglu P, Stutterd CA, Erasmus CE, Sanchez-Valle A, Lebon S, Hughes S, Schmitt-Mechelke T, Vasco G, Chow G, Rahikkala E, Dallabona C, Okuma C, Aiello C, Goffrini P, Abbink TEM, Bertini ES, Van der Knaap MS. Neurol Genet 7 e559 (2021)
  3. The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold. Das D, Kozbial P, Han GW, Carlton D, Jaroszewski L, Abdubek P, Astakhova T, Axelrod HL, Bakolitsa C, Chen C, Chiu HJ, Chiu M, Clayton T, Deller MC, Duan L, Ellrott K, Elsliger MA, Ernst D, Farr CL, Feuerhelm J, Grzechnik A, Grant JC, Jin KK, Johnson HA, Klock HE, Knuth MW, Krishna SS, Kumar A, Marciano D, McMullan D, Miller MD, Morse AT, Nigoghossian E, Nopakun A, Okach L, Oommachen S, Paulsen J, Puckett C, Reyes R, Rife CL, Sefcovic N, Tien HJ, Trame CB, van den Bedem H, Weekes D, Wooten T, Xu Q, Hodgson KO, Wooley J, Deacon AM, Godzik A, Lesley SA, Wilson IA. Acta Crystallogr Sect F Struct Biol Cryst Commun 66 1254-1260 (2010)


Reviews citing this publication (2)

  1. A fully integrated protein crystallization platform for small-molecule drug discovery. Hosfield D, Palan J, Hilgers M, Scheibe D, McRee DE, Stevens RC. J Struct Biol 142 207-217 (2003)
  2. Lessons from crystals grown in the Advanced Protein Crystallisation Facility for conventional crystallisation applied to structural biology. Vergara A, Lorber B, Sauter C, Giegé R, Zagari A. Biophys Chem 118 102-112 (2005)

Articles citing this publication (7)

  1. Non-discriminating and discriminating aspartyl-tRNA synthetases differ in the anticodon-binding domain. Charron C, Roy H, Blaise M, Giegé R, Kern D. EMBO J 22 1632-1643 (2003)
  2. Structural basis of the water-assisted asparagine recognition by asparaginyl-tRNA synthetase. Iwasaki W, Sekine S, Kuroishi C, Kuramitsu S, Shirouzu M, Yokoyama S. J Mol Biol 360 329-342 (2006)
  3. Molecular Dynamic Simulation of Space and Earth-Grown Crystal Structures of Thermostable T1 Lipase Geobacillus zalihae Revealed a Better Structure. Ishak SNH, Aris SNAM, Halim KBA, Ali MSM, Leow TC, Kamarudin NHA, Masomian M, Rahman RNZRA. Molecules 22 E1574 (2017)
  4. A microfluidic, high throughput protein crystal growth method for microgravity. Carruthers CW, Gerdts C, Johnson MD, Webb P. PLoS One 8 e82298 (2013)
  5. Comparison of the three-dimensional structures of a human Bence-Jones dimer crystallized on Earth and aboard US Space Shuttle Mission STS-95. Terzyan SS, Bourne CR, Ramsland PA, Bourne PC, Edmundson AB. J Mol Recognit 16 83-90 (2003)
  6. Fifty years excitement with science: recollections with and without tRNA. Giegé R. J Biol Chem 288 6679-6687 (2013)
  7. Crystallization and preliminary X-ray crystallographic study of a putative aspartyl-tRNA synthetase from the crenarchaeon Sulfolobus tokodaii strain 7. Suzuki K, Sato Y, Maeda Y, Shimizu S, Hossain MT, Ubukata S, Sekiguchi T, Takénaka A. Acta Crystallogr Sect F Struct Biol Cryst Commun 63 608-612 (2007)


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