1l5y Citations

Two-component signaling in the AAA + ATPase DctD: binding Mg2+ and BeF3- selects between alternate dimeric states of the receiver domain.

FASEB J 16 1964-6 (2002)
Cited: 37 times
EuropePMC logo PMID: 12368235

Abstract

A Crystallogral structure is described for the Mg2+-BeF3--bound receiver domain of Sinorhizobium meliloti DctD bearing amino acid substitution E121K. Differences between the apo- and ligand-bound active sites are similar to those reported for other receiver domains. However, the off and on states of the DctD receiver domain are characterized by dramatically different dimeric structures, which supports the following hypothesis of signal transduction. In the off state, the receiver domain and coiled-coil linker form a dimer that inhibits oligomerization of the AAA+ ATPase domain. In this conformation, the receiver domain cannot be phosphorylated or bind Mg2+ and BeF3-. Instead, these modifications stabilize an alternative dimeric conformation that repositions the subunits by approximately 20 A, thus replacing the a4-b5-a5 interface with an a4-b5 interface. Reoriented receiver domains permit the ATPase domain to oligomerize and stimulate open complex formation by the s54 form of RNA polymerase. NtrC, which shares 38% sequence identity with DctD, works differently. Its activated receiver domain must facilitate oligomerization of its ATPase domain. Significant differences exist in the signaling surfaces of the DctD and NtrC receiver domains that may help explain how triggering the common two-component switch can variously regulate assembly of a AAA+ ATPase domain.

Articles - 1l5y mentioned but not cited (7)

  1. Regulation of the transcriptional activator NtrC1: structural studies of the regulatory and AAA+ ATPase domains. Lee SY, De La Torre A, Yan D, Kustu S, Nixon BT, Wemmer DE. Genes Dev 17 2552-2563 (2003)
  2. Two variable active site residues modulate response regulator phosphoryl group stability. Thomas SA, Brewster JA, Bourret RB. Mol Microbiol 69 453-465 (2008)
  3. Matching biochemical reaction kinetics to the timescales of life: structural determinants that influence the autodephosphorylation rate of response regulator proteins. Pazy Y, Wollish AC, Thomas SA, Miller PJ, Collins EJ, Bourret RB, Silversmith RE. J Mol Biol 392 1205-1220 (2009)
  4. The aspartate-less receiver (ALR) domains: distribution, structure and function. Maule AF, Wright DP, Weiner JJ, Han L, Peterson FC, Volkman BF, Silvaggi NR, Ulijasz AT. PLoS Pathog 11 e1004795 (2015)
  5. Constitutive activation of two-component response regulators: characterization of VirG activation in Agrobacterium tumefaciens. Gao R, Mukhopadhyay A, Fang F, Lynn DG. J Bacteriol 188 5204-5211 (2006)
  6. A Variable Active Site Residue Influences the Kinetics of Response Regulator Phosphorylation and Dephosphorylation. Immormino RM, Silversmith RE, Bourret RB. Biochemistry 55 5595-5609 (2016)
  7. REC domain stabilizes the active heptamer of σ54-dependent transcription factor, FleR from Pseudomonas aeruginosa. Sahoo PK, Sheenu, Jain D. iScience 26 108397 (2023)


Reviews citing this publication (5)

  1. Bacterial response regulators: versatile regulatory strategies from common domains. Gao R, Mack TR, Stock AM. Trends Biochem Sci 32 225-234 (2007)
  2. The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription. Bush M, Dixon R. Microbiol Mol Biol Rev 76 497-529 (2012)
  3. Molecular strategies for phosphorylation-mediated regulation of response regulator activity. Gao R, Stock AM. Curr Opin Microbiol 13 160-167 (2010)
  4. Structures and organisation of AAA+ enhancer binding proteins in transcriptional activation. Schumacher J, Joly N, Rappas M, Zhang X, Buck M. J Struct Biol 156 190-199 (2006)
  5. Bacterial enhancer-binding proteins: unlocking sigma54-dependent gene transcription. Rappas M, Bose D, Zhang X. Curr Opin Struct Biol 17 110-116 (2007)

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