› 1lbh

X-ray diffraction
3.2Å resolution

INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG

Released:
DOI: 10.2210/pdb1lbh/pdb
PDB entry 1lbh coloured by chain, front view.
PDB entry 1lbh coloured by chain, side view.
PDB entry 1lbh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the lactose operon repressor and its complexes with DNA and inducer.
Lewis M, Chang G, Horton NC, Kercher MA, Pace HC, Schumacher MA, Brennan RG, Lu P
Science 271 1247-54 (1996)
PMID: 8638105

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-527190 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lactose operon repressor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 360 amino acids
Theoretical weight: 38.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-360; Coverage: 100%)
Gene names: JW0336, b0345, lacI
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand IPT 4 x IPT
No modified residues

Experiments and Validation Details

Entry percentile scores
Unit cell:
a: 141.2Å b: 75.1Å c: 149.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 not available
Expression system: Escherichia coli

Quick links

Citations

73 review citations

Intrinsically disordered protein.
Dunker et al. (2001)
72 more

11 mentions without citation

Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox Proteins.
Bondos et al. (2015)
10 more