› 1lk2

X-ray diffraction
1.35Å resolution

1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide

Released:
DOI: 10.2210/pdb1lk2/pdb
PDB entry 1lk2 coloured by chain, front view.
PDB entry 1lk2 coloured by chain, side view.
PDB entry 1lk2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects.
Rudolph MG, Shen LQ, Lamontagne SA, Luz JG, Delaney JR, Ge Q, Cho BK, Palliser D, McKinley CA, Chen J, Wilson IA, Eisen HN
J Immunol 172 2994-3002 (2004)
PMID: 14978103

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-525640 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Insulin receptor subunit alpha Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 934 Da
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P15208 (Residues: 423-430; Coverage: 1%)
Gene name: Insr

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
4 bound ligands:
Ligand NAG 1 x NAG
Ligand PO4 1 x PO4
Ligand MPD 2 x MPD
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21212
Unit cell:
a: 135.549Å b: 87.65Å c: 45.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.149 0.164
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

T cell allorecognition and MHC restriction--A case of Jekyll and Hyde?
Archbold et al. (2008)
1 more

8 mentions without citation

Crystallographic refinement of ligand complexes.
Kleywegt GJ. (2007)
7 more