› 1lkz

X-ray diffraction
2.5Å resolution

Crystal structure of D-ribose-5-phosphate isomerase (RpiA) from Escherichia coli.

Released:
DOI: 10.2210/pdb1lkz/pdb
PDB entry 1lkz coloured by chain, front view.
PDB entry 1lkz coloured by chain, side view.
PDB entry 1lkz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of D-ribose-5-phosphate isomerase (RpiA) from Escherichia coli.
Rangarajan ES, Sivaraman J, Matte A, Cygler M
Proteins 48 737-40 (2002)
PMID: 12211039

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 23.21 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z0 (Residues: 1-219; Coverage: 100%)
Gene names: JW5475, b2914, rpiA, ygfC
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2221
Unit cell:
a: 70.335Å b: 71.809Å c: 193.067Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.27
Expression system: Escherichia coli

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Citations

3 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
2 more

2 mentions without citation

Contribution of structural genomics to understanding the biology of Escherichia coli.
Matte et al. (2003)
1 more