› 1m07

X-ray diffraction
1.8Å resolution

RESIDUES INVOLVED IN THE CATALYSIS AND BASE SPECIFICITY OF CYTOTOXIC RIBONUCLEASE FROM BULLFROG (RANA CATESBEIANA)

Released:
DOI: 10.2210/pdb1m07/pdb
PDB entry 1m07 coloured by chain, front view.
PDB entry 1m07 coloured by chain, side view.
PDB entry 1m07 coloured by chain, top view.
Source organism: Aquarana catesbeiana
Primary publication:
Residues involved in the catalysis, base specificity, and cytotoxicity of ribonuclease from Rana catesbeiana based upon mutagenesis and X-ray crystallography.
Leu YJ, Chern SS, Wang SC, Hsiao YY, Amiraslanov I, Liaw YC, Liao YD
J Biol Chem 278 7300-9 (2003)
PMID: 12499382

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114159 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Oocytes ribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.46 KDa
Source organism: Lithobates catesbeianus
UniProt:
  • Canonical: P11916 (Residues: 23-133; Coverage: 100%)
Gene name: RCR
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain
5'-D(*AP*CP*GP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 nucleotides
Theoretical weight: 1.2 KDa
Source organism: Aquarana catesbeiana
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 28.629Å b: 53.566Å c: 72.203Å
α: 90° β: 98.31° γ: 90°
R-values:
R R work R free
0.227 0.189 0.228
Expression system: Not provided

Quick links

Citations

3 review citations

Ribonucleases as potential modalities in anticancer therapy.
Ardelt et al. (2009)
2 more

5 mentions without citation

Onconase and amphinase, the antitumor ribonucleases from Rana pipiens oocytes.
Ardelt et al. (2008)
4 more