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Aminoglycoside 2'-N-acetyltransferase from Mycobacterium tuberculosis in complex with coenzyme A and aminoglycoside substrates.

Vetting MW, Hegde SS, Javid-Majd F, Blanchard JS, Roderick SL
Nat Struct Biol 9 653-8 (2002)
Related entries: 1m44, 1m4g, 1m4i

Cited: 83 times
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Abstract

AAC(2')-Ic catalyzes the coenzyme A (CoA)-dependent acetylation of the 2' hydroxyl or amino group of a broad spectrum of aminoglycosides. The crystal structure of the AAC(2')-Ic from Mycobacterium tuberculosis has been determined in the apo enzyme form and in ternary complexes with CoA and either tobramycin, kanamycin A or ribostamycin, representing the first structures of an aminoglycoside acetyltransferase bound to a drug. The overall fold of AAC(2')-Ic places it in the GCN5-related N-acetyltransferase (GNAT) superfamily. Although the physiological function of AAC(2')-Ic is uncertain, a structural analysis of these high-affinity aminoglycoside complexes suggests that the enzyme may acetylate a key biosynthetic intermediate of mycothiol, the major reducing agent in mycobacteria, and participate in the regulation of cellular redox potential.

Reviews - 1m4d mentioned but not cited (4)

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Articles - 1m4d mentioned but not cited (3)

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Reviews citing this publication (17)

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Articles citing this publication (59)

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Related citations provided by authors (1)

  1. Overexpression and Mechanistic Analysis of Chromosomally Encoded Aminoglycoside 2'-N-Acetyltransferase (AAC(2')-Ic) from Mycobacterium tuberculosis. Hegde SS, Javid-Majd F, Blanchard JS J. Biol. Chem. 49 45876-45881 (2001)

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